Composite

Part:BBa_K4179009:Design

Designed by: Yasmin Habib   Group: iGEM22_Technion-Israel   (2022-10-05)


FcPT1 under Rhlr promoter + P2A + mCherry reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2218
    Illegal SpeI site found at 1006
    Illegal PstI site found at 1858
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1423
    Illegal SpeI site found at 1006
    Illegal PstI site found at 1858
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2218
    Illegal SpeI site found at 1006
    Illegal PstI site found at 1858
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2218
    Illegal SpeI site found at 1006
    Illegal PstI site found at 1858
    Illegal NgoMIV site found at 200
    Illegal NgoMIV site found at 765
    Illegal AgeI site found at 1497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 281
    Illegal SapI site found at 1077


Design Notes

The linker (BBa_K4179004) contains a 6xHis tag and a thrombin recognition and cleavage site.